Accession Number:

ADA609199

Title:

Zebrafish Model of NF1 for Structure-Function Analysis, Mechanisms of Glial Tumorigenesis, and Chemical Biology

Descriptive Note:

Annual rept. 1 May 2013-30 Apr 2014

Corporate Author:

DANA-FARBER CANCER INST BOSTON MA

Personal Author(s):

Report Date:

2014-05-01

Pagination or Media Count:

13.0

Abstract:

During the past year, we have continued to further our studies towards meeting the goals set forth in the funded proposal. An exciting phenotype has been identified that is relevant to the human NF1 disease and to the potential development of meaningful therapies. In collaboration with the Granato lab at Penn, the Epstein lab has discovered striking learning and memory defects in mutant fish. In the Look lab, the zebrafish models of the NF1 tumor suppressor linked cancers of MPNST and glioma have been completely updated to make them optimal for the structure function studies. Three different strategies are being applied to further accelerate the onset of these tumors to increase the precision of the structure function studies to elucidate the mechanism of tumor suppression by NF1. We have shipped our NF1 zebrafish lines to colleagues in the NF1 and zebrafish communities. These genetically engineered lines will be excellent candidates for use in screening small molecules and for use in performing genetic screens to identify modulators of this important disease.

Subject Categories:

  • Genetic Engineering and Molecular Biology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE