Understanding the Link Between Pregnancy and Breast Cancer
Annual rept. 1 Jun 2013-31 May 2014
ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI NEW YORK
Pagination or Media Count:
Our study is testing whether pregnancy affects the growth of breast cancer cells that expressed a mutant form of p53, which we found is responsible for the expression of PAPP-A. One of the hypothesis that was raised is that PAPP-A is a pregnancydependent oncogene. This year, we begun the characterization of the PAPP-A transgenic mice. The progress made led to a publication that is pending review. In short, PAPP-A was confirmed to be a pregnancy-dependent oncogene. We also made progress on the role of mutant p53. We found that the growth of genetically identical cells carrying a mutation in p53 is accelerated by the passage through pregnancy. The tumors volumes are bigger in the pregnancy group compared to the virgin group. We also found using microarray analysis that there are genes that are up-regulated specifically in the pregnancy group and not in the virgin group. We have begun the characterization of this pregnancy signature. We also obtained mice carrying a p53 mutant knockin in the p53 knockout mice background. We have established a colony and analyzed the effect of pregnancy on mammary tumor formation. So far we found only one mammary tumor in younger females. We are currently aging these mice. In addition, we have collected primary breast cancer sections and pregnancy history of breast cancer patients prior to their surgery. The analysis of this patient cohort is consistent with the data obtained in the PAPP-A transgenic mice and therefore the data is included in the same publication.
- Genetic Engineering and Molecular Biology
- Medicine and Medical Research