A Functional High-Throughput Assay of Myelination in Vitro
Final rept. 1 Jul 2012-30 Jun 2014
TULANE UNIV NEW ORLEANS LA
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Effective in vitro models of myelination, dysmyelination, andor remyelination would substantially speed the development and testing of potential therapies for myelin disorders such as multiple sclerosis. Tissues engineered from human induced pluripotent stem iPS may be effective at accurately modeling aspects of human physiology for screening of potential therapies. Highly enriched populations cells of neural lineage were obtained from human iPS cell lines that will serve as sources of microengineered human tissue. Hydrogel micropatterning methods were identified that may suppor the culture of human neurons and remain amenable to optical stimulation and recording. Methods were established for non-invasive monitoring of evoked electrophysiological responses in microengineered tissue constructs with voltage-sensitive dyes. This work represents a unique combination of enabling technologies, including human iPS cells, microfabrication, and optical neural recording. Though the defined tasks were not able to be fully completed, the capabilities and limitations of combining these technologies have been determined and challenges that must be overcome have been identified. The goal of producing a high-throughput, functional assay of human neural activity was demonstrated to be feasible.
- Medicine and Medical Research