Imaging Prostate Cancer with Positron Emission Tomography
Annual rept. 1 Sep 2013-31 Aug 2014
WAKE FOREST UNIV WINSTON-SALEM NC
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Tumors associated fibroblasts TAFs represent a major component of a PCa tumor, and play a critical role in tumor development. The purpose of this proposal is to utilize fibroblast activation protein alpha FAP expression on TAFs within the tumor stroma for the diagnostic imaging of PCa using novel radiopharmaceuticals and innovative multimodal imaging platforms. The scope of this proposal is to develop peptide based radiopharmaceuticals and evaluate them as PET imaging agents in preclinical animal models of prostate cancer, which demonstrate TAF involvement. This progress report describes the completed synthesis of these peptide ligands, their radiosynthesis with copper-64 and an evaluation of their stability in human serum. A second goal achieved during the current funding period was the development of fibroblast based cell lines, which stably express eGFP and FAP. Ongoing experiments are focused on the in vitro and in vivo evaluation of each radiopharmaceutical and on understanding the growth characteristics of each transfected cell line in vivo.
- Medicine and Medical Research