Pilot Study: Wound Healing with CorMatrix in the Rabbit (Oryctolagus cuniculus)
Final rept. 3 Jun 2013-15 Apr 2014
DAVID GRANT USAF MEDICAL CENTER TRAVIS AFB CA 60TH MEDICAL GROUP
Pagination or Media Count:
Objective Technique perfecting for wound healing model in the rabbit Results Using the five rabbits for technique perfecting proved very beneficial for the investigator. Multiple lessons were learned. 1. The CorMatrix covered the ear wounds best when they were made slightly larger than the wound. Originally, the ear wounds and CorMatrix patches were both cut with a 6mm biopsy punch. This made stitching the patch in place on all sides difficult. The patch was flush with the wound on one side and not necessarily on the opposite side. When the wound was made with a 5mm biopsy punch and the patch with the 6mm punch, the patch easily covered the entire wound on all sides when secured in place. 2. Four wounds per ear was optimum. More than four wound made it difficult if not impossible to avoid lacerating a large blood vessel or creating a wound proximal to the ear orifice an area difficult to place a wound dressing. It also made it difficult to maintain consistent spacing between each wound in an area of the ear that could have a dressing applied. 3. The original spacing between the wounds was 30 mm. That is much larger than necessary or desired. The 30mm distance made applying a dressing to cover all four wounds challenging. Making the wounds 10-15mm apart kept the wounds far enough apart to easily suture the treatment patches in place and not cross contaminate the controls. It also made it much easily to apply a dressing to the ears. 4. Each stitch required a minimum of four throws to tack the CorMatrix in place on four quadrants. The PDS suture has memory and easily unties when not adequately secured. In the first animals, only two throws per stitch were performed and the patches did not remain in place. Upon examination of corresponding histology slides there was no evidence of the CorMatrix patches. When four throws were used to secure the patches, the patch was verified by the histology results. 5.
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