NF1 Signal Transduction and Vascular Dysfunction
Annual rept. 1 May 2013-30 Apr 2014
ALBANY MEDICAL COLL NY
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We have made strong progress on each of the proposed Aims. We have successfully generated reagents for, and begun the evaluation of, several of the regulatory nodes that comprise the mTOR pathway including Rheb, Pten, Raptor, and Rictor. We have found that Rheb up- regulation weakly phenocopies NF1 loss, while PTEN loss shares many similar characteristics. We have found conditions to induce EndMT in primary endothelial cells. While data to date do not support the hypothesis that this is being modulated by the loss of NF1, this analysis is ongoing. Preliminary results suggest that the loss of NF1 may alter the balance of ALK receptor signaling and that the loss of ALK5 signaling can phenocopy the morphogenic defects seen with NF1 loss. Lastly we have successfully established the genetic lines required for our in vivo experiments and we are poised to begin the Cre-induction in the first experimental cohort.
- Anatomy and Physiology
- Medicine and Medical Research