Accession Number:

ADA605975

Title:

Induced Accelerated Aging in Induced Pluripotent Stem Cell Lines from Patients with Parkinson's Disease

Descriptive Note:

Final rept. 14 Nov 2011-13 Jun 2014

Corporate Author:

PARKINSONS INST SUNNYVALE CA

Personal Author(s):

Report Date:

2014-07-01

Pagination or Media Count:

20.0

Abstract:

The overall goal of the study was to develop a cell culture system from patient-derived induced pluripotent stem cells that expresses genes that can accelerate the aging process to facilitate modeling of neurodegenerative diseases such as Parkinson s disease. The objective of this study was to introduce the truncated lamin A gene, progerin, into patient-derived induced pluripotent stem cells using a doxycycline Dox-inducible expression system and differentiate them into dopaminergic neurons with the goal to accelerate the aging process of these cells and to promote a PD-related phenotype thus establishing a disease-in-a-dish model for PD. During our study, a publication from Lorenz Studer showed a very similar approach with lentiviral constructs of truncated lamin A in iPSC-differentiated neurons Miller et al. 2013. It shows that our hypothesis is correct and that the introduction of mutant forms of lamin A that can cause Hutchinson Gilford Progeria syndrome is accelerating the neurodegenerative phenotype in vitro.

Subject Categories:

  • Anatomy and Physiology
  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE