Micro and Nano-mediated 3D Cardiac Tissue Engineering
Annual rept. 24 Seo 2010-23 Sep 2011
ILLINOIS UNIV AT URBANA-CHAMPAIGN
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A grand challenge in cardiology since 1950s has been the development of an artificial heart that can replace a failing heart. This project envisages improving the care of battlefield-related cardiac injuries by providing novel methods to design and fabricate 3-D models of cardiac sub-components that would be critical in restoring the function of the heart. Our current research investigated the significance of geometrical constraints on the process of mammalian myogenesis. Using 3D stero-lithographic technology we developed a microvascular stamp that released multiple angiogenic growth factors and guided the formation of neovessels within patterns defined by the stamp. In order to test hydrogel constructs in vivo we developed a mouse myocardial infraction model. Using this model we have tested the creation, placement, and adhesion of patterned hydrogel heart patches. The fabrication process described in this work demonstrated for the first time, the ability to produce distributed feedback DFB biosensor surfaces uniformly over surface areas substantial enough to incorporate into standard format microplates. The fabrication was carried out in laboratory setting however, all methods are compatible with extension of the process to a roll-based manufacturing paradigm.
- Medicine and Medical Research