Micro and Nano-mediated 3D Cardiac Tissue Engineering
Annual rept. 24 Sep 2009 - 23 Sep 2010
ILLINOIS UNIV AT URBANA CENTER FOR NANOSCALE SCIENCE AND TECHNOLOGY
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The project envisages improving the care of battlefield-related cardiac injuries by providing novel methods to design and fabricate 3-D models of cardiac sub-components that would be critical in restoring the function of the heart. A commercially-available SLA was modified to accommodate two fabrication methods. We showed significant differences between myocytes extracted from atrium, ventricle and apex in vitro-culture. The results suggested that distinguishing the source of myocytes can be crucial for bioengineering myocardium and cell transplantation therapies. In addition, we successfully mobilized stem cells to the peripheral blood in less than 6 hours in the pig model using AMD-3100 Plerixafor injections. This new delivery method enables the slow release of an unstable protein-based therapeutic drug over a period of several days. In addition, we have developed a small animal model of myocardial infarction in order to test materials and methods for myocardial repair prior to deployment in a large animal model. A microvascular stamp was developed using microchannels with controlled diameter and spacing into a fibroblast-encapsulated hydrogel using a 3-D stereolithographic fabrication technique. The stamp created is likely to become a powerful tool to better understand vascular biology and improve clinical neovascularization. We also have demonstrated label-free imaging of cell attachment for a variety of cell types including panc-1, HepG2C3 and cardiomyocytes. The instrument has the ability to monitor changes in cell adhesion over a 24-hour period at the level of individual cells.
- Anatomy and Physiology