Novel Biomarker Discovery for Diagnostic and Therapeutic Strategies in Prostate Cancer
Annual rept. 1 Mar 2012-28 Feb 2013
MIAMI UNIV FL MILLER SCHOOL OF MEDICINE
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The purpose of this grant is to identify, isolate and characterize high affinity aptamers that distinguish between prostate cancers that are likely to remain organ-confined and those with potential to metastasize, The scope of this pilot is to generate DNA aptamers that selectively react with a prostate cancer cell line that remains confined to the prostate LNCaP vs. a subpopulation of this cell line that has acquired the ability to metastasize aggressively, employing Cell-Selex and Aptamer-Facilitated Biomarker Discovery AptaBiD technology. Major Findings and Progress 1 Non-metastatic LNCaP-Pro-5 cells and metastatic LNCaP-LN3 cells have been provided by Curtis Pettaway, M.D. Anderson Cancer Center, Houston, Texas. 2 Parental LNCaP cells have been obtained from American Type Culture Collection. 3 Phenotypic validation aggressive vs. indolent growth has been carried out on all cell lines using the Boyden Chamber assay. 4 DNA 40 bp aptamer libraries have been generated. 5 The first of 8 rounds of Cell-Selex has been initiated. 6 Plan for next-generation sequencing and bioinformatic analysis pathway has been established to yield desired phenotype-specific DNA aptamers. Next steps will include completion of Cell-Selex, NexGen sequencing and testing of DNA aptamer families on human clinical prostate cancer specimens.
- Medicine and Medical Research