Genetic and Functional Heterogeneity of Tumors in Neurofibromatosis 2
Annual rept. 15 Apr 2013-14 Apr 2014
MASSACHUSETTS GENERAL HOSPITAL BOSTON
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The intent of this project is to integrate whole exome genetic and global expression data to identify genes that contribute to the formation, progression and heterogeneity of NF2-associated tumors. To date we have prepared and submitted for exome sequencing at a sequencing core facility 126 samples representing paired human tumor meningioma or schwannoma and normal DNAs from the same individuals. We have also prepared RNA from the same tumors for transcriptome sequencing. Generation of these valuable datasets is on track for completion and subsequent individual and integrated analyses. In advance of the need to confirm the expected findings, we have generated a novel cell system using advanced genome editing technology that will permit the assessment of cellular phenotypes resulting from the genetic and gene expression changes identified.
- Anatomy and Physiology
- Medicine and Medical Research