Identification and Validation of Plasma Biomarkers in California Sea Lions
Final rept. 5 Sep 2007-4 Jan 2014
MEDICAL UNIV OF SOUTH CAROLINA CHARLESTON
Pagination or Media Count:
This proposal focused on the discovery and validation of serum or plasma biomarkers for domoic acid toxicosis DAT in sea lions. Proteomic approaches were utilized to assess whether proteins could classify sea lions as DAT or non-DAT. Commercial cytokine arrays and MALDI-T of peptide profiling can be utilized as screening tools in the diagnosis of acute DAT, but will only detect about 25 of those sea lions with DAT. 2D gel electrophoresis studies demonstrated that Apolipoprotein E and eosinophil counts combined resulted in a perfect marker of DAT in training set samples. External validation supported this combination as a biomarker with a test sensitivity of 86 and specificity of 85.