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Potential for Mosquitoes (Diptera: Culicidae) From Florida to Transmit Rift Valley Fever Virus
ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD VIROLOGY DIV
Pagination or Media Count:
We evaluated Aedes atlanticus Dyar and Knab, Aedes infirmatus Dyar and Knab, Aedes vexans Meigen, Anopheles crucians Wiedemann, Coquillettidia perturbans Walker, Culex nigripalpus Theobald, Mansonia dyari Belkin, Heinemann, and Page, and Psorophora ferox Von Humboldt from Florida to determine which of these species should be targeted for control should Rift Valley fever virus RVFV be detected in North America. Female mosquitoes that had fed on adult hamsters inoculated with RVFV were incubated for 7-21 d at 26 deg C, then allowed to refeed on susceptible hamsters, and tested to determine infection, dissemination, and transmission rates. We also inoculated mosquitoes intrathoracically, held them for 7 d, and then allowed them to feed on a susceptible hamster to check for a salivary gland barrier. When exposed to hamsters with viremias 10expn 7.6 plaque-forming units per milliliter of blood, at least some individuals in each of the species tested became infected however, Cx. nigripalpus, An. crucians, and Ae. infirmatus were essentially incompetent vectors in the laboratory because of either a midgut escape or salivary gland barrier. Each of the other species should be considered as potential vectors and would need to be controlled if RVFV were introduced into an area where they were found. Additional studies need to be conducted with other geographic populations of these species and to determine how environmental factors affect transmission.
APPROVED FOR PUBLIC RELEASE