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Bioassay-Guided Investigation of Two Monarda Essential Oils as Repellents of Yellow Fever Mosquito Aedes aegypti
MISSISSIPPI UNIV UNIVERSITY NATIONAL CENTER FOR NATURAL PRODUCTS RESEARCH
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As part of an ongoing research program to identify active mosquito repellents, Monarda bradburiana Beck and Monarda fistulosa L. essential oils showed good repellent activity with minimum effective dosages MED of 0.055 0.036 and 0.078 0.027 mgcm2, respectively, compared to reference standard N,N-diethyl-3-methylbenzamide DEET 0.039 0.014 mgcm2. Systematic bioassay-guided fractionation of essential oils of both Monarda species was performed to identify the active repellent compounds, and isolated pure compounds were individually tested for repellency. Of the isolated compounds, carvacrol, thymol, eugenol, and carvacrol methyl ether were found to be the repellent compounds with MEDs in the range of 0.013 0.063 mgcm2. Active repellent compounds were also tested for larvicidal activity against 1-day-old Aedes aegypti larvae. Thymol was the best larvicide among the tested individual compounds LD50 of 13.9 ppm. None of the individual compounds showed cytotoxicity against mammalian cells however, the essential oils were toxic to all cell lines.
APPROVED FOR PUBLIC RELEASE