Accession Number:

ADA599371

Title:

Whole Adult Organism Transcriptional Profiling of Acute Metal Exposures in Male Zebrafish

Descriptive Note:

Research 1 Jan 2007-3 Jan 2014

Corporate Author:

ARMY CENTER FOR ENVIRONMENTAL HEALTH RESEARCH FORT DETRICK MD

Report Date:

2014-03-10

Pagination or Media Count:

16.0

Abstract:

A convergence of technological breakthroughs in the past decade has facilitated the development of rapid screening tools for biomarkers of toxicant exposure and effect. Platforms using the whole adult organism to evaluate the genome-wide response to toxicants are especially attractive. Recent work demonstrates the feasibility of this approach in vertebrates using the experimentally robust zebrafish model. In the present study, we evaluated gene expression changes in whole adult male zebrafish following an acute 24 hr high dose exposure to three metals with known human health risks. Male adult zebrafish were exposed to nickel chloride, cobalt chloride or sodium dichromate concentrations corresponding to their respective 96 hr LC20, LC40 and LC60. Histopathology was performed on a subset of metal-exposed zebrafish to phenotypically anchor transcriptional changes associated with each metal.

Subject Categories:

  • Biology
  • Toxicology
  • Inorganic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE