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Neuroprotection and Anti-Epileptogenesis with a Mitochondria-Targeted Antioxidant
Annual rept. 30 Sep 2012-29 Sep 2013
RESEARCH FOUNDATION FOR MENTAL HYGIENE INC STATEN ISLAND NY
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The goals of the project were to assess the neuroprotective and antiepiletogenic properties of a mitochondrial-targeted antioxidant, SS-31 using the pilocarpine Pilo model of status epilepticus SE, the kindling seizure model and the tetanus toxin Tx model. Progress focused on Aim1 due to limited access to SS-31. For Aim 2 the kindling model was established in the laboratory in preparation for drug testing. For Aim1, adult male Sprague-Dawley rats 260-405g were pretreated with SS-31 3 or 10mgkg, sc n14 or saline n10 45min before induction of SE with Pilo 365mgkg, sc. The latency to SE onset was measured for both groups. The brains were fixed 1-3 days after SE, sectioned through the hippocampus and stained for Nissl, Fluoro-jade C FJ, NeuN and heat shock protein HSP. Three rats from each group died during SE. There was no significant difference in the latency to SE between the two groups. There was also no difference in Nissl, FJ, or NeuN staining between the groups. However one day after SE there was an increase in HSP staining in the granule cells of the dentate gyrus. These results suggest that SS-31 is not neuroprotective in the Pilo model but may be effective against less severe insults to the brain.
APPROVED FOR PUBLIC RELEASE