Accession Number:

ADA599245

Title:

Hetero-oligonucleotide Nanoscale Tiles Capable of Two-Dimensional Lattice Formation as Testbeds for a Rapid, Affordable Purification Methodology

Descriptive Note:

Journal article

Corporate Author:

SAINT JOHN'S UNIV JAMAICA NY

Personal Author(s):

• Lukeman, Philip S.

Report Date:

2013-01-01

Pagination or Media Count:

6.0

Abstract:

New nanoscale hetero-oligonucleotide tiles are assembled from DNA, RNA and morpholino oligos and purified using size exclusion filtration. Homo-oligonucleotide tiles assembled from RP-cartridge processed DNA oligos are purified by nondenaturing gel electro- phoresis. These tiles purity and homogeneity are demonstrated by gel electrophoresis and their incorporation into two-dimensional arrays visualized by AFM. This purification methodology increases throughput and decreases costs for researchers who wish to screen multiple tiles for utilization in structural or analytical studies.

Descriptors:

• *ARRAYS
• *DEOXYRIBONUCLEIC ACIDS
• *NANOTECHNOLOGY
• *NUCLEOTIDES
• *PURIFICATION
• ELECTROPHORESIS
• FILTRATION
• RIBONUCLEIC ACIDS
• TEST BEDS
• TILES
• TWO DIMENSIONAL

Subject Categories:

• Biochemistry
• Industrial Chemistry and Chemical Processing
• Organic Chemistry
• Electrical and Electronic Equipment

Distribution Statement:

APPROVED FOR PUBLIC RELEASE