Accession Number:

ADA599213

Title:

Rapid Dispersion of Polymicrobial Wound Biofilms with Depolymerase Enzymes

Descriptive Note:

Final rept. 4 Oct 2010-3 Oct 2013

Corporate Author:

MARYLAND UNIV COLLEGE PARK

Personal Author(s):

Report Date:

2013-11-01

Pagination or Media Count:

42.0

Abstract:

During the award period, we successfully developed protocols to extract biofilm EPS associated with ESKAPE organisms Enterococcus, Staphylococcus, Klebsiella, Acinetobacter, Pseudomonas, and Enterobacter species. We further performed composition and linkage analysis on these samples and determined that 2 and 3-linked mannose residues constitute the major carbohydrate moiety in the EPS. In parallel, we synthesized, expressed, purified, and characterized 15 putative depolymerase enzymes by a variety of assays against static, dynamic, and mixed biofilm conditions. At least eight enzymes displayed significant anti-biofilm activity and three NagZ, DspNW, and Betty were advanced to animal safety testing.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE