Determining the Location of DNA Modification and Mutation Caused by UVB Light in Skin Cancer
Annual summary 15 Aug 2012-14 Aug 2013
COLORADO UNIV AURORA CO
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Extrinsic modifications to the DNA bases such as pyrimidine dimers can arise from a variety of exposures and can lead to aberrant cell growth or death. A detailed view of this base modification is necessary for a more complete view of genetic and epigenetic regulation but the process is poorly understood. We are developing a method to look at the precise genomic position of these modifications using a next generation sequencing approach. During this research period we have been performing preliminary experiments to aid in streamlining the processes that will be used in this research. We have purified or obtained the enzymes needed for aim 1 of this research. Using these enzymes we have shown that we obtain cleavage patterns consistent with the administered UV dosage and that sequencing libraries generated for both yeast and human cells show pyrimidine bias on the 5 end, indicating that we are sequencing the dimers. Understanding where these modifications occur is a critical first step to understanding the mutations they cause.
- Anatomy and Physiology
- Medicine and Medical Research