Biomarkers in the Detection of Prostate Cancer in African Americans
Annual rept. 1 Sep 2012-31 Aug 2013
TUFTS-NEW ENGLAND MEDICAL CENTER BOSTON MA
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The focus of this proposal is to identify molecular markers in high grade prostate cancer PCa based on biopsies, especially from African Americans AAs who usually select radiation rather than radical prostatectomies. How these differ molecularly from European Americans EAs is of major interest. All IRBs and MTAs have been approved at participating sites. Dr. Gaston s laboratory has extracted DNA from 756 nitrocellulose blots from 63 patients including 40 AAs and racial admixtures have been determined on 12 of these cases 2 out of 12 had significant differences from self declared race and DNA studies have been extended to paraffin blocks. Multiplex immunoasay pilot studies have been performed on serum, plasma and urine. Molecules of interest that are relatively elevated in PCa include EGF, sFAS2, CEA, G-CSF, il-13, and MIP-1 . Molecules that are relatively decreased include free PSA, He-4, il-1a, il-1b, and TGF . Work with Dr. Clayton Yates has identified microRNAs that are differentially expressed in PCa and show racial differences including microRNA-152 which correlated with aggressive features. Also, epigenetic field effects induced by PCa have been identified.
- Anatomy and Physiology
- Medicine and Medical Research