Repeated-Dose and Reproductive/Developmental Toxicity of NTO (3-Nitro-1,2,4-Triazol-5-One) in the Rat
Technical rept. Apr-Jul 2012
ARMY PUBLIC HEALTH COMMAND ABERDEEN PROVING GROUND MD ARMY INST OF PUBLIC HEALTH
Pagination or Media Count:
Male and female Sprague-Dawley rats were administered NTO in corn oil 0, 31.25, 125, and 500 mgkg-day via oral gavage for two weeks prior to mating and for an additional two weeks during mating. Female rats were dosed throughout gestation and for four days following birth. Two male recovery groups 0 and 500 mgkg-day were held for four weeks following the four-week dosing period. Reductions in testes and epididymal mass and mass ratios were observed in the 500 mgg-day group compared to controls. Sperm counts were also reduced in the 500 mgkg-day group compared to controls with no motile sperm observed. The reductions in testes and epididymal mass were associated with tubular degenerationatrophy and hypospermia with cribiform change of the epididymides. Complete recovery was not evident in the 500 mgkg-day recovery group. No discernable differences were recognized between dose groups for the reproductive parameters monitored. Gross external examinations of the offspring did not indicate that NTO presents a developmental hazard.