Imaging Prostate Cancer Microenvironment by Collagen Hybridization
Annual rept. 30 Sep 2012-29 Sep 2013
JOHNS HOPKINS UNIV BALTIMORE MD
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We continue to derivatize and test the collagen mimetic peptides CMPs for imaging. Our proposed milestones in year two include 1 Dual radio- and fluorescent labeling of CMPs retaining high-affinity Kd 10-8 M and specificity for intact and digested collagen type I films 2 Validation of dual-labeled CMPs that display high affinity and specificity for stromal collagens in frozen PCa xenografts and, 3 Measurement of pharmacokinetics and in vivo imaging of dual-labeled CMPs in mouse subcutaneous PC-3 xenograft models. Efforts have been focused on achieving a biologically stable CMP endowed with both gamma-emitting and fluorescent labels milestone 1. To that end, we have employed several strategies utilizing the previously biologically validated, high affinity basic CMP peptide core, which may contain chelators, covalently bound radioiodine moieties and fluorescent dyes.
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