The Dynamics of Intracellular Uptake of Surface Modified Poly (d, L-Lactide-co-Glycolide) Nanoparticles in Breast Cancer Cells (Rev)
Final rept. 25 Aug 2003-24 Aug 2004
HAMPTON UNIV VA
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Internalization by cells of macromolecular drugs and drug loaded nanoparticles can be most advantageous in overcoming several limitations in cancer prevention and treatment. These systems are generally considered to be impermeable to cell membranes. However, internalization has been observed to occur via endocytosis. Affinity for cell membrane and high uptake is improved by several mechanisms including increased hydrophobicity, presence of surface charge or presence of targeting moieties in macromolecules or on nanoparticles. Specific targeting of deranged epithelial cells in the breast with biodegradable, controlled drug release nanoparticle systems is an attractive approach to solving problems or prevention and treatment of breast cancer. In this study, we sought to demonstrate that nanoparticles coated with an epidermal growth factor EGF would be preferentially internalized in MCF-7 breast cancer cells. Optimal nanoparticle sizes ranging in size of 120 200 nm were obtained using acetone and polyvinyl alcohol PVA. Freeze dried nanoparticles were readily re-suspendable. MCF-7 cells were incubated with EGF-coated and non-coated nanoparticles. Observation cells by confocal microscopy after incubation with coated and non-coated nanoparticles failed to reveal nanoparticle internalization by MCF-7 cells.
- Medicine and Medical Research