Accession Number:

ADA595708

Title:

In Vivo Multimodal Imaging of NF-kappaB (pB) Spatial and Temporal Activation Following Light Injury in the Mouse Retina

Descriptive Note:

Interim rept. Jan 2010-Jan 2012

Corporate Author:

AIR FORCE RESEARCH LAB FORT SAM HOUSTON TX HUMAN PERFORMANCE WING (711TH) HUMAN EFFECTIVENESS DIR/ DIRECTED ENERGY BIOEFFECTS DIVISION

Report Date:

2014-01-15

Pagination or Media Count:

46.0

Abstract:

BALBc and C57BL6 wild type mice and cis-NF- B-EGFP transgenic mouse model were used to perform in vivo experiments of laserinduced thermal damage to the retina. A Heidelberg Spectralis HRA confocal scanning laser ophthalmoscope with a spectral domain optical coherence tomographer was used to obtain fundus and cross-sectional images of laser induced injury in the retina. Sub-threshold, threshold, and supra-threshold lesions were imaged up to 10 days post exposure using optical coherence tomography OCT, infrared reflectance, red-free reflectance, fluorescence angiography, and autofluorescence imaging modalities. The spatial and temporal activation of Nuclear factor-kappa B NF- B p65 was monitored in the retina of the cis-NF- B-EGFP mouse. In addition, EGFP basal expression in brain and retinal tissue from the cis-NF- B-EGFP was characterized using two-photon imaging. Injured and control eyes were enucleated at discrete time points following laser exposure for cryosectioning and confocal microscopy to determine localization of NF- B dependent enhanced green fluorescent protein EGFP reporter gene expression within the retina. Furthermore, immunohistochemistry for GFAP, IBA-1, NeuN, CD65, and glutamine synthetase were used to examine the retinal structures and cell population expressing upregulated activation of p65 as a result of laser induced injury.

Subject Categories:

  • Anatomy and Physiology
  • Radiation and Nuclear Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE