Rapid Parallel Screening for Strain Optimization
Quarterly rept. 17 Nov 2013-16 Feb 2014
J CRAIG VENTER INST ROCKVILLE MD
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Progress has been made on identifying biosensors that will be used to report on the fermentation yields of industrially relevant biological compounds. Screening of the desired chemicals, sequencing and annotation of isolated microbes was completed previously. Construction and optimization of reporter systems for testing candidate transcription factors was completed previously. Similarly, we have optimized the screening process so that empirical verification of transcription factor candidates is less labor intensive than originally envisioned. During this period we have finalized the identification of biodegradation gene clusters likely to be controlled by the desired transcription factors. Nearly 40 high quality candidates have been identified. DNA sequences representing transcription factors and their corresponding operators will now be assembled directly into the reporter system. Upon identification of a suitable and well-behaved transcription factor, the biosynthetic pathway for the corresponding biochemical will be installed and we will begin work on overproducing the molecule.
- Genetic Engineering and Molecular Biology
- Miscellaneous Detection and Detectors