The Snail-Induced Sulfonation Pathway in Breast Cancer Metastasis
Annual rept. 1 Jul 2012-30 Jun 2013
PENNSYLVANIA UNIV PHILADELPHIA WISTAR INST
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This project seeks to establish a key role for cellular sulfonation enzymes in the metastatic progression of breast cancer. To this end we have accomplished the following tasks in this second year of support 1. Further characterization of Tetracycline inducible WT Snail and mutant Snail vectors and cell lines. 2. Demonstration that the PAPSS2 knockdown vectors do not work andor are very leaky and must be further developed. 3. Demonstration MCF-10A cells undergo dramatic morphologic EMT in the presence of WT Snail expression, with the downregulation of the Cell adhesion molecule E-cadherin and the upregulation of the mesenchymal markers vimentin and fibronectin. 4. Characterization of antibodies to PAPSS1 and PAPSS2 enzymes and demonstration that they cross react with one another making their use in IHC very problematic. 5. Request for, and granting of an extension of this research project in order to solve the unexpected technical problems.
- Medicine and Medical Research
- Organic Chemistry