tRNA and Its Activation Targets as Biomarkers and Regulators of Breast Cancer
Final rept. 1 Sep 2010-31 Aug 2013
CHICAGO UNIV IL
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Transfer RNAs tRNAs are small non-coding RNAs that read the genetic code in protein synthesis. They are essential for the proliferation, fitness, and adaptation of the cell. Previously, we showed that elevated tRNA levels are characteristic of breast cancer cells Pavon-Eternod, M., Gomes, S., Geslain, R., Dai, Q., Rosner, M.R. and Pan, T. 2009 tRNA over-expression in breast cancer and functional consequences. Nucleic Acids Res, 37, 7268-7280. Furthermore, overexpression of one specific tRNA - the initiator methionine tRNA - leads to increased cell proliferation and altered tRNA expression in a non-cancer breast epithelial cell line. Based on these results, we hypothesized that tRNA over-expression alters the translational regulation of key genes involved in cancer development and progression. We aim to identify the protein or RNA targets that are mis-regulated upon tRNA over-expression, and to determine the effect of tRNA over-expression on tumor initiation and progression.
- Anatomy and Physiology
- Medicine and Medical Research