Protocol Development and Preliminary Toxicity Study of CBRN Nanomaterials
Final rept. Jan-Sep 2012
ARMY PUBLIC HEALTH COMMAND ABERDEEN PROVING GROUND MD ARMY INST OF PUBLIC HEALTH
Pagination or Media Count:
This project uses fast, high-throughput in vitro approach to study new nanomaterials applicable to Chemical Biological Defense CBD for their environmental and human related toxicity. Four CBD nanomaterials were evaluated for their aquatic toxicity by Microtox test and human cytotoxicity by NRU assay. These studies fill the data gaps and provide toxicity information useful in risk assessment when the environmental and human exposure to these new CBD nanomaterials. In addition, Transepithelial Electrical Resistance TER and Transepithelial Permeability TEP assays were developed and tested on EpiAirway, a 3-D human trachealbronchial epithelial equivalent. Further evaluation of the effects of these CBD nanomaterials on tissue tight junction and barrier function is the on-going study. This project supports the efforts of USAPHCOTSG in CBRN incident response for protecting the work force, civilian and military, from the unintended consequences of nanotechnology processes and materials exposure.