Identifying the Mechanism(s) Responsible for the Translational Regulation of the Stress Signaling Kinase MKK4
Annual rept. 1 Aug 2011-31 Jul 2012
CHICAGO UNIV IL
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Since our last progress report we have focused on developing the cell lines and in vivo approaches that are needed to complete the work proposed in Aim 2. We have also revised our SOW accordingly to reflect these and other changes required to best achieve our outcomes. As described in the last report, we were particularly interested in the potential use of the CWR22Rv1 prostate cancer cell line, which recapitulates key aspects of clinical disease. In the same light, we extended our studies into the C4-2B cell line, which has also been reported to be bone-seeking and potentially metastasis-forming. Given that our goal is to test the effect of MKK4 levels on metastatic colonization, it was imperative that we first focus our efforts on 1 confirming the reported metastatic ability of both the CWR22Rv1 and C4-2B cell lines, and 2 optimizing the conditions and approach for quantitative evaluation of the effect of modulating MKK4 protein levels on bone metastasis formation after intracardiac injection.
- Medicine and Medical Research