Accession Number:

ADA591563

Title:

Dignital Biological Converter

Descriptive Note:

Corporate Author:

SYNTHETIC GENOMICS INC LAJOLLA CA

Personal Author(s):

Report Date:

2014-01-03

Pagination or Media Count:

6.0

Abstract:

SGI has previously reported completion of the first three milestones. In this report we describe the successful demonstration of the fourth milestone fully automated generation of protein starting from a DNA sequence that codes for that protein. In our example, we produced green fluorescence protein GFP. We entered a 1.5-kb gene sequence for GFP into SGIs Archetype that automatically generated a file containing the overlapping oligonucleotides necessary to produce the 1.5-kb gene fragment and initiated the start of the Digital-Biological Converter DBC run. Forty-eight overlapping 64-base oligonucleotide oligonucleotides were synthesized, post-processed, and then pooled. The pooled oligonucleotides were then assembled and the resulting GFP gene fragment was amplified, error-corrected, and then re-amplified. This synthetic GFP gene product was then incubated in a cell-free extract and the synthetic GFP sequence was translated into protein. Multiple reactions were carried out in parallel and as much as 37 gmL GFP protein was produced.

Subject Categories:

  • Biochemistry
  • Genetic Engineering and Molecular Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE