Mammary Cancer and Activation of Transposable Elements
Annual rept. 1 Sep 2012-31 Aug 2013
ADELAIDE UNIV (AUSTRALIA)
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The purpose of this project is to investigate molecular events occurring in the preclinical stages of mammary cancer. Specifically, the project investigates the intersection between the development of genome demethylation, retrotransposon transcriptional activity, and retrotransposon-driven transcription of cellular genes in an engineered mouse model of mammary cancer. During the last 12 months, mouse breeding for the project was completed and collection of material for planned molecular analyses was delayed but is now almost complete. Preparation of RNA and DNA for molecular library construction is nearly complete, construction of molecular libraries is underway in Australia and with my collaborator Dr Edwards at Washington University in St Louis. Library analyses have been initiated in Dr Edwards laboratory. We believe that the eventual findings will provide insights into understanding the role of genome hypomethylation and expression of retrotransposons in cancer ontogeny, and may impact cancer prevention in the future.
- Anatomy and Physiology
- Medicine and Medical Research