Inhibition of Breast Cancer Progression by Blocking Heterocellular Contact between Epithelial Cells and Fibroblasts
Final rept. 1 Apr 2011-30 Mar 2012
WISCONSIN UNIV MADISON
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In breast cancer progression, the transition from ductal carcinoma in situ DCIS to invasive ductal carcinoma IDC is a life-threatening step. This step is accompanied by a dramatic drop in prognosis. Stromal fibroblasts and epithelial cells are separated by a basement membrane BM at the early DCIS stage. However, once the BM is disrupted and stromal invasion of epithelial cells is initiated, direct heterocellular contacts between fibroblasts and epithelial cells often occurs. This suggests that the signaling through the heterocellular contact may be a crucial factor in how the invasive progress continues after it is initiated. Accordingly, the objective of this proposal is to investigate the influence of heterocellular contacts between MCF-DCIS cells and human mammary fibroblasts HMFs in breast cancer progression by employing a microfluidic-based compartmentalized 3D co-culture platform enabling both contact-free and contact-associated co-cultures.
- Anatomy and Physiology
- Medicine and Medical Research