Cell Lineage Analysis of Mouse Prostate Carcinogenesis
Annual rept. 1 Jul 2012-30 Jun 2013
PITTSBURGH UNIV PA
Pagination or Media Count:
Defining the origin of prostate cancer cells is fundamentally important and will guide future research to focus on cells from which prostate cancer cells are derived. Prostate cancer is thought to be derived from luminal epithelial cells in the prostate, because a hallmark of prostate cancer is the loss of basal epithelial cells and prostate cancer cells exhibit a luminal epithelial cell phenotype including the expression of AR and PSA. However, the luminal origin of prostate cancer has been challenged by a number of recent publications. This project will determine whether prostate cancer cells are derived from luminal or basal epithelial cells in an EAF2-- PTEN- mouse model, and determine whether luminal-derived prostate cancer cells behave differently from basal-derived prostate cancer cells. In the last year of the funding period, we have generated 1 breeding pair consisting of 1 male and 1 female of PSA-CreERT2 R26RmTmGEAF2-PTEN- mice for Specific Aim 1. Also, we have obtained CK14-CreERT2 mice and began breeding to generate CK14-CreERT2 R26RmTmGEAF2--PTEN- mice for Specific Aim 2.
- Anatomy and Physiology
- Medicine and Medical Research