Accession Number:

ADA588546

Title:

Phylogenetic and Metabolic Diversity of Hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX)-transforming Bacteria in Strictly Anaerobic Mixed Cultures Enriched on RDX as Nitrogen Source

Descriptive Note:

Journal article

Corporate Author:

AIR FORCE RESEARCH LAB TYNDALL AFB FL

Report Date:

2003-01-01

Pagination or Media Count:

9.0

Abstract:

Five obligate anaerobes that were most closely related to Clostridium bifermentans, Clostridium celerecrescens, Clostridium saccharolyticum, Clostridium butyricum and Desulfovibrio desulfuricans by their 16S rRNA genes sequences were isolated from enrichment cultures using hexahydro-1,3,5-trinitro-1,3,5-triazine RDX as a nitrogen source. The above isolates transformed RDX at rates of 24.0, 5.4, 6.2, 2.5, 5.5 micro molecule h-1 g dry weight of cells-1, respectively, to nitrite, formaldehyde, methanol, and nitrous oxide. The present results indicate that clostridia are major strains responsible for RDX removal, and all isolates seemed to mainly transform RDX via its initial reduction to MNX and subsequent denitration. Since clostridia are commonly present in soil, we suggest that they may contribute to the removal of RDX in the subsurface anoxic soil.

Subject Categories:

  • Microbiology
  • Inorganic Chemistry
  • Ammunition and Explosives

Distribution Statement:

APPROVED FOR PUBLIC RELEASE