Accession Number:

ADA588422

Title:

Development of Toxicity Benchmarks for Nitrogen-based Energetic Materials for the Enchytraeid Worm, Enchytraeus crypticus

Descriptive Note:

Final rept. Apr 2004-Dec 2012

Corporate Author:

ARMY EDGEWOOD CHEMICAL BIOLOGICAL CENTER APG MD

Report Date:

2013-11-01

Pagination or Media Count:

58.0

Abstract:

We investigated individual toxicities of nitrogen-based energetic materials EMs 2,4-dinitrotoluene 2,4-DNT, 2-amino-3,6-dinitrotoluene 2-ADNT, 4-amino-2,6-dinitrotoluene 4-ADNT, nitroglycerin NG, and octahydro-1,3,5,7-tetranitro-1,3,5,7-tetrazocine HMX to the potworm Enchytraeus crypticus using the Enchytraeid Reproduction Test ISO 163872004. Studies were designed to generate ecotoxicological benchmarks for developing the ecological soil screening levels Eco-SSLs for risk assessments of contaminated soils, and to identify and characterize the predominant soil physicochemical parameters that affect the toxicity of 2,4-DNT to E. crypticus. Soils with a wide range of physicochemical parameters included Teller sandy loam, Sassafras sandy loam, Kirkland loam, and Webster clay loam. Reproduction EC50 values mg kg 1 for EMs weathered-and-aged W-A in sandy loam soils were 27 2,4-DNT, 37 4-ADNT, 103 2-ADNT, 146 NG, and 10,208 HMX. Reproduction toxicity of 2,4-DNT W-A in soil, based on EC50 values, correlated strongly with soil organic matter and clay contents. Toxicity benchmarks established in TSL and SSL have been submitted to the U.S. Environmental Protection Agency Eco-SSL Workgroup for use in developing soil invertebrate-based Eco-SSLs for 2,4-DNT, 2-ADNT, 4-ADNT, and NG.

Subject Categories:

  • Biology
  • Toxicology
  • Geology, Geochemistry and Mineralogy

Distribution Statement:

APPROVED FOR PUBLIC RELEASE