Rapid Parallel Screening for Strain Optimization
Quarterly rept. 17 May-16 Aug 2013
J CRAIG VENTER INST ROCKVILLE MD
Pagination or Media Count:
Progress has been made on identifying biosensors that will be used to report on the fermentation yields of industrially relevant biological compounds. Screening of the desired chemicals was completed previously. Microbes that can utilize these compounds as their sole carbon source have been isolated and archived. Genomic DNA from 97 isolates was prepared for sequencing last quarter. Sixty-nine samples were submitted to sequencing and sixty-five of those passed quality controls and have been sequenced and assembled. Genome annotation is ongoing. About 10 of the sequenced genomes have been fully annotated and more are completed each day. Bioinformatic analysis of completed genomes has provided candidate transcription factors based on their localization adjacent to or within appropriate catabolic gene clusters. Experimental work has produced and verified two vectors for screening these transcription factors and clusters 1 a modular assembly vector with a reporter, and, 2 a yeast TAR cloning shuttle vector for transferring catabolic clusters to E. coli.
- Genetic Engineering and Molecular Biology