Development and Characterization of Novel Bioluminecent Systems
Final rept. 1 May 2010-30 Apr 2013
CONNECTICUT COLL NEW LONDON
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The overall objective of this project was to discover, design and demonstrate the feasibility of bioluminescent materials for use in marking, tagging, and anti-tamper applications.1 The technology for near-Infrared nIR dye labeling and BRET activation of a firefly luciferase were transferred to Rajesh Naiks lab at AFRL.2 The construction and evaluation of novel protease substrates luciferase fusion proteins emitting nIR light for caspase and the two blood clotting factors was completed.3 Novel nIR emitting quantum dotsrods were made using luciferases in collaboration with Mathew Maye, Syracuse University. 4 A firefly luciferin benzothiophene-containing analog with glow kinetics and 523 nm emission was discovered. 5 Analyses of luminescent materials from C. variopedatus provided by the Deheyn lab revealed the presence of riboflavin.6 A firefly luciferase variant was intramolecularly cross-linked trapping it in a C-domain rotated previously undocumented conformation. 7 With A. Gulick Hauptman Woodward Institute luciferase crystal structures were determined providing insight into the mechanism of the oxidative light reaction.