Accession Number:

ADA586109

Title:

Nanofluidic Lab-On-Chip Technology for DNA Identification

Descriptive Note:

Final technical rept. May 2012-Jun 2013

Corporate Author:

CALIFORNIA UNIV REGENTS SANTA BARBARA OFFICE OF RESEARCH

Personal Author(s):

Report Date:

2013-09-30

Pagination or Media Count:

24.0

Abstract:

In this project we have investigated the potential of nanofluidic lab-on-chip technology to be used as platforms for free-flow separation, concentration, and identification of biomolecules. Specifically, the goals of this project have been to 1 demonstrate Field Amplified Sample Stacking FASS for specific DNA samples, and 2 demonstrate identification of target single stranded DNA ssDNA from a complex mixture of DNA, containing strands of different composition and size. We have achieved both goals and, in some cases, expanded the investigation from the original tasks. The results of our studies, elaborated in the following sections, suggest that lab-on-chip nanofluidic platforms may enable rapid and inexpensive, characterization and analysis of DNA biomarkers. Advantages include overall ease of operation of the device, which does not require loading of gels as sieving matrices inexpensive analysis, as the only reagents required are water-based buffers fast identification, since sample signatures can be produced in the order of less than a minute. Findings from this work have resulted in peer-reviewed publications one conference and one journal paper. Potential target applications of our identification platform include analysis of DNA biomarkers for environmental pollutants or early disease diagnosis analysis of mitochondrial DNA for forensic identification investigation of protein kinetics.

Subject Categories:

  • Biochemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE