Accession Number:

ADA585495

Title:

Purification and Characteriztion of the Type III Secretion System Protein from Burkholderia mallei

Descriptive Note:

Final rept. Jan-Oct 2010

Corporate Author:

ARMY EDGEWOOD CHEMICAL BIOLOGICAL CENTER APG MD

Report Date:

2013-08-01

Pagination or Media Count:

20.0

Abstract:

Burkholderia mallei is a Gram-negative zoonotic pathogen of solipeds that can also secondarily infect human hosts causing glanders. The bacterium is highly infectious and has been considered in the past as a potential biological weapon. In Western Europe and North America, glanders has been eradicated, but it persists in other areas. The two major bacterial virulence systems, the type III T3SS and type VI T6SS secretion systems, have been studied and found to be essential for the virulence in animal models of the disease. B. mallei has two T3SSs that are plant-like and animal-like, but only the latter is essential for virulence in animal models of glanders. In this study, we have expressed, purified, and measured the catalytic activity of the B. mallei BsaS ATPase in an in vitro assay.

Subject Categories:

  • Medicine and Medical Research
  • Microbiology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE