Vitamin D Pathway Status and the Identification of Target Genes in the Mouse Mammary Gland
Revised annual summary rept. 1 Jan-31 Dec 2012
STATE UNIV OF NEW YORK AT ALBANY
Pagination or Media Count:
Mammary gland samples were isolated from wild type, vitamin D receptor knockout VDRKO and 1alphahydroxylase knockout 1 KO female mice for whole mounts and paraffin embedding inguinal and for RNA and protein isolation thoracic. Time points collected included 6-10wk old nulliparous, 9 and 16 days pregnancy, 5 and 10 days lactation and 3 and 6 days involution. All whole mounts were completed and showed increased branching during pregnancy in the VDRKO glands relative to wild type and 1 KO glands. Paraffin embedded involution samples were stained with hematoxylin and eosin and showed an apparent decrease in alveolar breakdown in the first few days of involution in VDRKO and 1 KO glands compared to wild type controls. These results suggest that 1 KO mammary glands do not develop exclusively similar to VDRKO or wild type glands which verifies our need to complete the remainder of our studies to determine if the VDR is acting to control mammary gland development through the vitamin D pathway or through some other ligand or possibly without a ligand.
- Medicine and Medical Research