Accession Number:

ADA583966

Title:

Morphological and Functional Differentiation in BE (2)-M17 Neuroblastoma Cells by Treatment with Trans-Retinoic Acid

Descriptive Note:

Journal article

Corporate Author:

ARMY MEDICAL RESEARCH INST OF CHEMICAL DEFENSE ABERDEEN PROVING GROUND MD

Report Date:

2013-04-18

Pagination or Media Count:

13.0

Abstract:

Background Immortalized neuronal cell lines can be induced to differentiate into more mature neurons by adding specific compounds or growth factors to the culture medium. This property makes neuronal cell lines attractive as in vitro cell models to study neuronal functions and neurotoxicity. The clonal human neuroblastoma BE2-M17 cell line is known to differentiate into a more prominent neuronal cell type by treatment with trans-retinoic acid. However, there is a lack of information on the morphological and functional aspects of these differentiated cells. Results We studied the effects of trans-retinoic acid treatment on a some differentiation marker proteins, b types of voltage-gated calcium Ca2 channels and c Ca2-dependent neurotransmitter 3H glycine release in cultured BE2-M17 cells. Cells treated with 10 M trans-retinoic acid RA for 72 hrs exhibited marked changes in morphology to include neurite extensions presence of PQ, N and T-type voltage-gated Ca2 channels and expression of neuron specific enolase NSE, synaptosomal-associated protein 25 SNAP-25, nicotinic acetylcholine receptor 7 nAChR- 7 and other neuronal markers. Moreover, retinoic acid treated cells had a significant increase in evoked Ca2-dependent neurotransmitter release capacity. In toxicity studies of the toxic gas, phosgene CG, that differentiation of M17 cells with RA was required to see the changes in intracellular free Ca2 concentrations following exposure to CG. Conclusion Taken together, retinoic acid treated cells had improved morphological features as well as neuronal characteristics and functions thus, these retinoic acid differentiated BE2-M17 cells may serve as a better neuronal model to study neurobiology andor neurotoxicity.

Subject Categories:

  • Anatomy and Physiology
  • Organic Chemistry

Distribution Statement:

APPROVED FOR PUBLIC RELEASE