Novel Strategy for Lineage Tracing of Cancer Stem Cells by Lentiviral Barcoding
Annual summary rept. 15 Sep 2009-14 Sep 2012
BAYLOR COLL OF MEDICINE HOUSTON TX
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The cancer stem cell theory posits a subpopulation of tumorigenic cells with self-renewal potential and the capability to wholly reconstitute original tumors by lineage restriction. We proposed a novel lentiviral tagging system to genetically trace immunosorted cell fate, and further investigate relative TIC enrichment through immunosorting protocols. We have developed a genetic-based strategy to dramatically improve sensitivity and experimental time in prospective identification of novel biomarkers. In months 25-36, we build on our previous results and implemented next-generation sequencing methodology to further trave the tumor sublineages in the mouse mammary tumor model.
- Medicine and Medical Research