Accession Number:

ADA583773

Title:

Novel Strategy for Lineage Tracing of Cancer Stem Cells by Lentiviral Barcoding

Descriptive Note:

Annual summary rept. 15 Sep 2009-14 Sep 2012

Corporate Author:

BAYLOR COLL OF MEDICINE HOUSTON TX

Personal Author(s):

Report Date:

2012-10-01

Pagination or Media Count:

10.0

Abstract:

The cancer stem cell theory posits a subpopulation of tumorigenic cells with self-renewal potential and the capability to wholly reconstitute original tumors by lineage restriction. We proposed a novel lentiviral tagging system to genetically trace immunosorted cell fate, and further investigate relative TIC enrichment through immunosorting protocols. We have developed a genetic-based strategy to dramatically improve sensitivity and experimental time in prospective identification of novel biomarkers. In months 25-36, we build on our previous results and implemented next-generation sequencing methodology to further trave the tumor sublineages in the mouse mammary tumor model.

Subject Categories:

  • Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE