A Functional High-Throughput Assay of Myelination in Vitro
Annual rept. 1 Jul 2012-30 Jun 2013
TULANE UNIV NEW ORLEANS LA
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Effective in vitro models of myelination, dysmyelination, andor remyelination would substantially speed the development and testing of potential therapies for myelin disorders such as multiple sclerosis. Tissues engineered from human induced pluripotent stem iPS may be effective at accurately modeling aspects of human physiology for screening of potential therapies. Substantial progress has been made toward demonstrating the feasibility of developing microengineered human neural tissues that can be assessed non-invasively. A population of neurons has been derived from human iPS cell lines, a micropatterned hydrogel culture system has been identified for the support of human neurons, and an optical method for monitoring physiological responses in microengineered tissue constructs has been demonstrated. This works represents a unique combination of enabling technologies, including human iPS cells, microfabrication, and optical neural recording, with the goal of showing how it may be possible to create a high-throughput assay of human neural activity.
- Medicine and Medical Research
- Stress Physiology