Potentiometric Detection of Pathogens
Final rept. 5 Sep 2009 - 4 Sep 2012
POLYTECHNIC INST OF NEW YORK BROOKLYN
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The project started with a goal of developing microfluidic single working electrode device using open circuit potentiometry as the transduction method. The uniqueness was based on the high surface area of the nanosize organic electrode conducting polymer top-layer surface. This approach has then been changed to the gate modification in ion sensitive field effect transistors, in which our unique approach contains floating gate approach to make the operation more simple. Presently our FGFET-arrays floating gate ion sensitive field effect transistor arraysconsist of encapsulated 8x8 64 gate arrays, and the next designs have been completed for 16x64 and 64x64 arrays to monitor changes in analytes with a large number of proteins. Antigen binding is monitored using capture antibody or capture imprint and both validated with monoclonal antibody conjugated with HRP enzyme which acts as a charge-amplifier. The final project report describes first the development of the conducting polymer top-layer, which makes the devices very functional and competitive. Secondly, the device development is discussed and finally the preclinical results are presented with a main focus on the recent development of cardiac marker panel with five different markers. As the use of semiconductor technology for DNA sequencing has induced a major change in the field of sequencing, we pioneer similar trends for protein-protein interaction studies. Continuous monitoring of over one thousand protein-protein interactions will yield novel information of disease progression especially in its early stages.
- Physical Chemistry
- Miscellaneous Detection and Detectors