Accession Number:

ADA583552

Title:

Inactivation of Spores of Bacillus Species by Wet Heat: Studies on Single Spores Using Laser Tweezers Taman Spectroscopy

Descriptive Note:

Final rept. 1 Nov 2008-31 Oct 2012

Corporate Author:

EAST CAROLINA UNIV GREENVILLE NC

Personal Author(s):

Report Date:

2013-02-01

Pagination or Media Count:

10.0

Abstract:

We have investigated the aspects of the release of Ca-dipicolinic acid DPA, protein denaturation and cellular heterogeneity of single Bacillus spores during wet-heat treatment that are commonly used in spore killing and inactivation. Achievements include 1 determined kinetic change in spore state and Ca-DPA levels in single spores of Bacillus and Clostridium species during heat activation 2 measured the rates of Ca-DPA release and protein denaturation of individual spores when incubated in water at elevated temperatures 3 determined the effects of wet heat-treatment on the germination of individual spores and identified the damages of key proteins 4 developed useful methodologies to analyze multiple individual spores during wet-heat treatment and germination.

Subject Categories:

  • Biology

Distribution Statement:

APPROVED FOR PUBLIC RELEASE