Inactivation of Spores of Bacillus Species by Wet Heat: Studies on Single Spores Using Laser Tweezers Taman Spectroscopy
Final rept. 1 Nov 2008-31 Oct 2012
EAST CAROLINA UNIV GREENVILLE NC
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We have investigated the aspects of the release of Ca-dipicolinic acid DPA, protein denaturation and cellular heterogeneity of single Bacillus spores during wet-heat treatment that are commonly used in spore killing and inactivation. Achievements include 1 determined kinetic change in spore state and Ca-DPA levels in single spores of Bacillus and Clostridium species during heat activation 2 measured the rates of Ca-DPA release and protein denaturation of individual spores when incubated in water at elevated temperatures 3 determined the effects of wet heat-treatment on the germination of individual spores and identified the damages of key proteins 4 developed useful methodologies to analyze multiple individual spores during wet-heat treatment and germination.