Role of NuSAP in Prostate Tumor Progression
Annual rept. 15 May 2012-14 May 2013
STANFORD UNIV CA
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We have identified NuSAP as prognostic marker up-regulated in recurrent prostate tumors. Our grant aimed at identifying the role of NuSAP in promoting proliferation and invasion in Prostate Cancer and identify genes that upregulate NuSAP expression. To characterize the role and regulation of NuSAP in prostate cancer, we expanded our investigation into looking at critical regulatory elements within the NuSAP promoter region. We identified 2 non-canonical MYC binding sites within the 1000bp of NuSAP promoter. Luciferase expression studies using wildtype and deletion constructs suggested no direct response of MYC on NuSAP promoter region. We also carried out studies to understand whether NuSAP expression is regulated by RB1E2F1 axis. We found NuSAP expression increased upon knockdown of RB1 and a further reduction of E2F1 resulted in reduction of NuSAP gene and protein suggesting that NuSAP is regulated by the RB1E2F1 axis.
- Medicine and Medical Research