Accession Number:

ADA582839

Title:

Construction and Nonclinical Testing of a Puumala Virus Synthetic M Gene-Based DNA Vaccine

Descriptive Note:

Journal article

Corporate Author:

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD VIROLOGY DIV

Personal Author(s):

• Brocato, R. L.
• Josleyn, M. J.
• Wahl-Jensen, V.
• Schmaljohn, C. S.
• Hooper, J. W.

Report Date:

2012-12-12

Pagination or Media Count:

11.0

Abstract:

Puumala virus PUUV is a causative agent of hemorrhagic fever with renal syndrome HFRS. Although PUUV-associated HFRS does not result in high case-fatality rates, the social and economic impact is considerable. There is no licensed vaccine or specific therapeutic to prevent or treat HFRS. Here we report the synthesis of a codon-optimized, full-lengthMsegment open reading frame and its cloning into a DNA vaccine vector to produce the plasmid pWRGPUU-Ms2. pWRGPUU-Ms2 delivered by gene gun produced high-titer neutralizing antibodies in hamsters and nonhuman primates. Vaccination with pWRG PUU-Ms2 protected hamsters against infection with PUUV but not against infection by related HFRS-associated hantaviruses. Unexpectedly, vaccination protected hamsters in a lethal disease model of Andes virus ANDV in the absence of ANDV crossneutralizing antibodies. This is the first evidence that an experimental DNA vaccine for HFRS can provide protection in a hantavirus lethal disease model.

Descriptors:

• *DEOXYRIBONUCLEIC ACIDS
• *HEMORRHAGIC FEVERS
• *INFECTIOUS DISEASES
• *VACCINES
• CLONES
• FEVERS
• GENES
• IMMUNIZATION
• KIDNEYS
• SIGNS AND SYMPTOMS
• SYNTHESIS
• VECTOR ANALYSIS
• VIRUSES

Subject Categories:

• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE