Accession Number:

ADA582383

Title:

Epigenetic Testing for Breast Cancer Risk Stratification

Descriptive Note:

Annual rept. 15 Sep 2011-14 Sep 2012

Corporate Author:

TEXAS UNIV SOUTHWESTERN MEDICAL SCHOOL AT DALLAS

Personal Author(s):

• Euhus, David

Report Date:

2012-10-01

Pagination or Media Count:

13.0

Abstract:

DNA methylation is increasingly recognized as an early molecular change in benign epithelium that is implicated in the development of high risk preneoplasia and cancer. Using a genome-wide screen we have previously identified methylation markers optimized for RP-FNA samples with potential for tissue-based breast cancer risk stratification. Most methylation markers are most relevant to ER-POS breast cancer. We have thoroughly assessed 12 new markers in an archival RP-FNA and cancer FNA sample set derived from 180 women. VCAN was identified as a gene preferentially methylated in ER-POS breast cancer and IRF7 as a gene preferentially methylated in ER-NEG breast cancer. We have now assembled a 7 marker panel with good coverage of ER-POS and ER-NEG breast cancer for assessment in prospectively acquired RP-FNA and cancer FNA samples. In addition, validation of an RP-FNA-based approach to risk stratification has been hampered by the lack of an unselected unaffected control cohort. We are rapidly expanding a community-based RP-FNA sample repository that provides an ideal unselected unaffected control cohort for validation of our panel in the coming year.

Descriptors:

• *BREAST CANCER
• *EPITHELIUM
• *STRATIFICATION
• DEOXYRIBONUCLEIC ACIDS
• GENES
• MARKERS
• METHYLATION
• RISK
• SAMPLING

Subject Categories:

• Medicine and Medical Research

Distribution Statement:

APPROVED FOR PUBLIC RELEASE