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Transplantation of Reprogrammed Autologous Stem Cells for Chronic Pain and Drug Abuse
Annual rept. 30 Sep 2011-29 Sep 2012
ILLINOIS UNIV AT CHICAGO
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This project is a Partnering PI option with Dr. Jianguo Cheng at CCF as Initiating PI and Dr. Tingyu Qu at UIC as Partnering PI. The specific aims are to generate functional chromaffin-like cells from mesenchymal stem cells MSCs and to investigate the analgesic and anti-tolerance effects and the safety of chromaffin-like cells in animal models. We have conducted the proposed experiments as outlined in SOW. Specifically, we have produced chromaffin-like cells by reprogramming human MSCs hMSCs with the extracts of porcine adrenal chromaffin cells. We have harvested bone marrow tissues from rats and isolated, cultured, and expanded rat MSCs rMSCs for the targeted reprogramming by using cellular extracts of porcine adrenal chromaffin cells to produce chromaffin-like cells. Recently, we found that even na ve MSCs at their early passages passage 5 had analgesic effects in animals and had profound inhibitory effects on the development of morphine tolerance in a SH-SY5Y cellular model. Our research has led to one manuscript in press, one poster presentation, and one abstract in this first year of a 3 year project.
APPROVED FOR PUBLIC RELEASE