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Search and Neutralize Factors (CSPGs) that Induce Decline in Transmission to Neurons from Spared Fibers after Chronic Spinal Cord Injury
Annual rept. 30 Sep 2011-29 Sep 2012
ITL RESEARCH CORP NORTHRIDGE CA
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During Year 2 we have used anti-NG2-antibodyes made in Dr. Levines lab and accomplished following studies. 1 Experiments described in Specific Aim SA2 we demonstrate that a single intraspinal injection of anti-NG2- antibody Ab removes conduction block and restores transmission deficits induced by acute NG2. 2 Experiments described in SA3 and SA4 related to chronic effects of NG2-Ab delivered via osmotic minipump we demonstrate that prolonged infusion of anti-NG2-Ab strengthens transmission, promotes anatomical plasticity SA3 and improves locomotor function and coordination in automated Catwalk gait SA4 after chronic hemisection SCI. 3 We have succeeded to create the recombinant single chain scFv antibody and confirmed expression of anti-NG2- Ab at present, AAV10 encoding the anti-NG2 scFv is being constructed in PENN vector core. During year 3 we will examine functional effects of this AAV-NG2-Ab construct, and accomplish SA3 and 4. If successful, this AAV-NG2- Ab construct will be the novel effective and clinically relevant treatment to facilitate recovery after SCI. Four papers have been published during 2011-2012 period two other papers have been submitted for publication.
APPROVED FOR PUBLIC RELEASE